Kāore i te ōrite te tohatoha o te ua i te Porowini o Guizhou i ngā wā o te tau, he nui ake te ua i te kōanga me te raumati, engari ka pāngia ngā tipu rēpī e te ahotea tauraki i te ngahuru me te takurua, ka pā kino ki te hua. He hua hinu motuhake te māti e whakatipuria ana i te Porowini o Guizhou. He kaha tōna ātete ki te tauraki, ā, ka taea te whakatipu i ngā wāhi maunga. He rauemi nui o ngā ira ātete-tauraki. He mea tino nui te kitenga o ngā ira ātete-tauraki mō te whakapai ake i ngā momo māti, me te auahatanga i roto i ngā rauemi germplasm. He mahi nui tā te whānau GRF i roto i te tipu me te whanaketanga o ngā tipu me te urupare ki te ahotea tauraki. I tēnei wā, kua kitea ngā ira GRF i roto i te Arabidopsis 2, te raihi (Oryza sativa) 12, te rēpī 13, te miro (Gossypium hirsutum) 14, te witi (Triticum), te aestivum)15, te miro peara (Setaria italica)16 me te Brassica17, engari kāore he pūrongo mō ngā ira GRF i kitea i roto i te māti. I roto i tēnei rangahau, i tautuhia ngā ira whānau GRF o te māti i te taumata whānui o te ira, ā, i tātarihia ō rātou āhuatanga ā-tinana, ā-matū hoki, ngā whanaungatanga whanaketanga, te ōritetanga, ngā motika kua tiakina, te hanganga ira, ngā tāruarua ira, ngā huānga-cis me te wāhanga tipu (wāhanga rau-whā). I tātarihia whānuitia ngā tauira whakaputanga i raro i te ahotea tauraki hei whakarato i tētahi pūtake pūtaiao mō ētahi atu rangahau mō te mahi pea a ngā ira BjGRF i roto i te urupare tauraki, me te whakarato i ngā ira kaitono mō te whakatipu māti ātete-tauraki.
E toru tekau mā whā ngā ira BjGRF i kitea i roto i te ira Brassica juncea mā te whakamahi i ngā rapunga HMMER e rua, kei roto katoa i ēnei ko ngā rohe QLQ me WRC. Kei roto i te Ripanga Tāpiri S1 ngā raupapatanga CDS o ngā ira BjGRF kua kitea. Kua tapaina a BjGRF01–BjGRF34 i runga i tō rātou wāhi i runga i te ira. E tohu ana ngā āhuatanga ā-tinana me te matū o tēnei whānau he tino rerekē te roa o te waikawa amino, mai i te 261 aa (BjGRF19) ki te 905 aa (BjGRF28). Ko te pūwāhi isoelectric o BjGRF kei waenganui i te 6.19 (BjGRF02) ki te 9.35 (BjGRF03) me te toharite o te 8.33, ā, 88.24% o BjGRF he pūmua taketake. Ko te awhe taumaha ngota i matapaetia o BjGRF kei waenganui i te 29.82 kDa (BjGRF19) ki te 102.90 kDa (BjGRF28); Ko te taupū kore pumau o ngā pūmua BjGRF kei waenganui i te 51.13 (BjGRF08) ki te 78.24 (BjGRF19), he nui ake i te 40 katoa, e tohu ana ko te taupū waikawa ngako kei waenganui i te 43.65 (BjGRF01) ki te 78.78 (BjGRF22), ko te waiaroaro toharite (GRAVY) kei waenganui i te -1.07 (BjGRF31) ki te -0.45 (BjGRF22), he kino ngā uara GRAVY o ngā pūmua BjGRF waiaroaro katoa, tērā pea nā te kore o te waiaroaroaro i puta mai i ngā toenga. I whakaatuhia e te matapae taunga pūtau-iti e 31 ngā pūmua kua whakakōrerohia e te BjGRF ka taea te taunga ki roto i te karihi, ka taea te taunga ki roto i ngā peroxisome te BjGRF04, ka taea te taunga ki roto i ngā cytoplasm te BjGRF25, ā, ka taea te taunga ki roto i ngā chloroplast te BjGRF28 (Ripanga 1), e tohu ana ka taea te taunga ki roto i te karihi ngā BjGRF, ā, he mahi whakahaere nui tā rātou hei take whakawhiti.
Mā te tātaritanga ira-ira o ngā whānau GRF i roto i ngā momo rerekē ka āwhina i te ako i ngā mahi ira. Nō reira, i tangohia ngā raupapatanga waikawa amino roa-katoa o te 35 o te kāpeti rēpī, 16 o te tānipa, 12 o te raihi, 10 o te millet me te 9 o ngā Arabidopsis GRF, ā, i hangaia he rākau ira-ira i runga i te 34 o ngā ira BjGRF kua tautuhia (Pikitia 1). He rerekē te maha o ngā mema o ngā whānau iti e toru; 116 ngā GRF TF kua wehea kia toru ngā whānau iti rerekē (ngā rōpū A~C), kei roto ko te 59 (50.86%), 34 (29.31%) me te 23 (19.83)% o ngā GRF. I roto i a rātou, e 34 ngā mema o te whānau BjGRF kua marara puta noa i ngā whānau iti e 3: 13 ngā mema i roto i te rōpū A (38.24%), 12 ngā mema i roto i te rōpū B (35.29%) me te 9 ngā mema i roto i te rōpū C (26.47%). I roto i te tukanga o te polyploidization o te māti, he rerekē te maha o ngā ira BjGRF i roto i ngā whānau iti rerekē, ā, tera pea kua puta te whakanui me te ngaronga o ngā ira. He mea tika kia mōhiotia kāore he tohatoha o ngā GRF raihi me te rai i roto i te rōpū C, engari e rua ngā GRF raihi me te kotahi te GRF rai i roto i te rōpū B, ā, ko te nuinga o ngā GRF raihi me te rai kua whakarōpūhia ki te peka kotahi, e tohu ana he whanaunga tata ngā BjGRF ki ngā dicots. I roto i ēnei, ko ngā rangahau hohonu rawa atu mō te mahi GRF i roto i te Arabidopsis thaliana e whakarato ana i te pūtake mō ngā rangahau mahi o ngā BjGRF.
He rākau whakawhanaunga o te māti tae atu ki te Brassica napus, te Brassica napus, te raihi, te rai me ngā mema o te whānau Arabidopsis thaliana GRF.
Te tātari i ngā ira tāruarua i roto i te whānau GRF māti. Ko te rārangi hina i te papamuri e tohu ana i tētahi poraka tukutahi i roto i te ira māti, ko te rārangi whero e tohu ana i tētahi takirua tāruarua wāhanga o te ira BjGRF;
Te whakaaturanga ira BjGRF i raro i te ahotea tauraki i te wāhanga rau tuawhā. Kei te whakaatuhia ngā raraunga qRT-PCR i te Ripanga Tāpiri S5. Ko ngā rerekētanga nui o ngā raraunga e tohuhia ana e ngā reta iti.
I te haere tonu o te huringa o te āhuarangi o te ao, kua noho hei kaupapa rangahau wera te ako i te āhua o te tu atu a ngā hua ki te ahotea tauraki me te whakapai ake i ō rātou tikanga manawanui18. Whai muri i te tauraki, ka rerekē te hanganga āhua, te whakaaturanga ira me ngā tukanga pākia o ngā tipu, ka mutu pea te photosynthesis me te raruraru pākia, ka pā ki te hua me te kounga o ngā hua19,20,21. Ina rongo ngā tipu i ngā tohu tauraki, ka puta he karere tuarua pērā i a Ca2+ me te phosphatidylinositol, ka whakanui ake i te kukū ion konupūmā ā-roto, ka whakahohe i te whatunga whakahaere o te ara phosphorylation pūmua22,23. Ko te pūmua whāinga whakamutunga e whai wāhi tika ana ki te tiaki pūtau, e whakahaere rānei i te whakaaturanga o ngā ira ahotea e pā ana mā roto i ngā TF, ka whakarei ake i te manawanui o ngā tipu ki te ahotea24,25. Nō reira, he mahi nui tā ngā TF ki te urupare ki te ahotea tauraki. E ai ki te raupapatanga me ngā āhuatanga here DNA o ngā TF e aro ana ki te ahotea tauraki, ka taea te wehewehe i ngā TF ki ngā whānau rerekē, pērā i a GRF, ERF, MYB, WRKY me ētahi atu whānau26.
Ko te whānau ira GRF he momo TF motuhake ki te tipu e whai wāhi nui ana ki ngā āhuatanga maha pēnei i te tipu, te whanaketanga, te tuku tohu me ngā urupare tiaki tipu27. Mai i te kitenga o te ira GRF tuatahi i roto i te O. sativa28, kua piki haere te maha o ngā ira GRF kua kitea i roto i te maha o ngā momo, ā, kua whakaatuhia he pānga ki te tipu tipu, te whanaketanga me te urupare ahotea8, 29, 30,31,32. Nā te whakaputanga o te raupapatanga ira Brassica juncea, ka taea te tautuhi i te whānau ira BjGRF33. I roto i tēnei rangahau, e 34 ngā ira BjGRF i kitea i roto i te ira māti katoa, ā, i tapaina ko BjGRF01–BjGRF34 i runga i tō rātou tūranga ira. Kei roto katoa i ēnei rohe QLQ me WRC tino tiakina. I whakaatuhia e te tātaritanga o ngā āhuatanga ā-tinana me te matū kāore i te nui ngā rerekētanga o ngā tau waikawa amino me ngā taumaha ngota o ngā pūmua BjGRF (haunga te BjGRF28), e tohu ana he rite tonu ngā mahi a ngā mema o te whānau BjGRF. I whakaatuhia e te tātaritanga hanganga ira e 64.7% o ngā ira BjGRF he 4 ngā exon, e tohu ana he āhua tiakina te hanganga ira BjGRF i roto i te whanaketanga, engari he nui ake te maha o ngā exon i roto i ngā ira BjGRF10, BjGRF16, BjGRP28 me BjGRF29. Kua whakaatuhia e ngā rangahau ko te tāpiri, te muku rānei i ngā exon, i ngā intron rānei ka arahi ki ngā rerekētanga o te hanganga me te mahi a te ira, ā, ka hangaia he ira hou34,35,36. Nō reira, e whakapae ana mātou i ngaro te intron o BjGRF i te wā whanaketanga, ka puta pea he huringa ki te mahi a te ira. E rite ana ki ngā rangahau o nāianei, i kitea hoki e mātou ko te maha o ngā intron e pā ana ki te whakaaturanga ira. Ina nui te maha o ngā intron i roto i te ira, ka taea e te ira te urupare wawe ki ngā āhuatanga kino.
Ko te tāruarua ira he mea nui i roto i te whanaketanga ira me te ira37. Kua whakaatuhia e ngā rangahau e pā ana ko te tāruarua ira ehara i te mea ka whakanui ake i te maha o ngā ira GRF anake, engari he huarahi hoki ki te whakaputa i ngā ira hou hei āwhina i ngā tipu ki te urutau ki ngā āhuatanga taiao kino38. E 48 ngā takirua ira tāruarua i kitea i roto i tēnei rangahau, he tāruarua wāhanga katoa ēnei, e tohu ana ko ngā tāruarua wāhanga te tikanga matua mō te whakanui ake i te maha o ngā ira i roto i tēnei whānau. Kua pūrongohia i roto i ngā tuhinga ka taea e te tāruarua wāhanga te whakatairanga i te whakanui ake i ngā mema o te whānau ira GRF i roto i te Arabidopsis me te rōpere, ā, kāore i kitea he tāruarua takirua o tēnei whānau ira i roto i tētahi o ngā momo27,39. He rite ngā hua o tēnei rangahau ki ngā rangahau o nāianei mō ngā whānau Arabidopsis thaliana me te rōpere, e kī ana ka taea e te whānau GRF te whakanui ake i te maha o ngā ira me te whakaputa i ngā ira hou mā te tāruarua wāhanga i roto i ngā tipu rerekē.
I roto i tēnei rangahau, e 34 ngā ira BjGRF i kitea i roto i te māti, ā, i wehea kia 3 ngā whānau iti. I whakaatuhia e ēnei ira he rite tonu ngā motika me ngā hanganga ira kua tiakina. I whakaatuhia e te tātaritanga hononga-ira e 48 ngā takirua o ngā tāruatanga wāhanga i roto i te māti. Kei roto i te rohe whakatairanga BjGRF ngā huānga mahi-cis e pā ana ki te urupare mārama, te urupare homoni, te urupare ahotea taiao, me te tipu me te whanaketanga. I kitea te whakaaturanga o ngā ira BjGRF e 34 i te wāhanga tipu māti (pakiaka, kakau, rau), me te tauira whakaaturanga o ngā ira BjGRF e 10 i raro i ngā āhuatanga tauraki. I kitea he rite tonu ngā tauira whakaaturanga o ngā ira BjGRF i raro i te ahotea tauraki, ā, tera pea he rite tonu te whai wāhi ki te whakahaere i te kaha o te tauraki. Tērā pea he mahi whakahaere pai tā ngā ira BjGRF03 me BjGRF32 i roto i te ahotea tauraki, ko BjGRF06 me BjGRF23 he mahi i roto i te ahotea tauraki hei ira ūnga miR396. I te nuinga, ka whakaratohia e tā mātou rangahau he pūtake koiora mō te kitenga ā muri ake nei o te mahi ira BjGRF i roto i ngā tipu Brassicaceae.
Nā te Guizhou Oil Seed Research Institute, Guizhou Academy of Agricultural Sciences ngā purapura māti i whakamahia i tēnei whakamātautau i whakarato. Tīpakohia ngā purapura katoa ka whakatō ki te oneone (whakatakoto: oneone = 3:1), ka kohia ngā pakiaka, ngā kakau me ngā rau i muri i te wāhanga rau-whā. I rongoatia ngā tipu ki te 20% PEG 6000 hei whakatauira i te tauraki, ā, i kohia ngā rau i muri i te 0, 3, 6, 12 me te 24 hāora. I tio tonutia ngā tauira tipu katoa ki te hauota wai, kātahi ka rongoatia ki roto i te pouaka tio -80°C mō te whakamātautau e whai ake nei.
Kei roto i te tuhinga kua whakaputaina me ngā kōnae mōhiohio tāpiri ngā raraunga katoa i kohia, i tātaritatia rānei i tēnei rangahau.
Wā tuku: Hanuere-22-2025