E whakaatu ana tēnei rangahau ko te harore e hono ana ki te pakiaka, ko Kosakonia oryziphila NP19, i wehea mai i ngā pakiaka raihi, he patu pepeke koiora me te matū koiora whai hua hei whakahaere i te pahūtanga raihi. I whakahaerehia ngā whakamātautau in vitro ki ngā rau hou o ngā tipu raihi kakara Khao Dawk Mali 105 (KDML105). I whakaatu ngā hua i te whai huatanga o te aukati a te NP19 i te whakatipu o ngā konidia harore pahūtanga raihi. I aukatia te mate harore i raro i ngā tikanga maimoatanga rerekē e toru: te werohanga raihi ki te NP19 me ngā konidia harore; te werohanga rau i te wā kotahi ki te NP19 me ngā konidia harore; me te werohanga rau ki ngā konidia harore, ā, i muri mai ka rongoatia ki te NP19 30 hāora. I tua atu, i whakaitihia e te NP19 te tipu o te hyphae harore mā te 9.9–53.4%. I roto i ngā whakamātautau kohua, i whakanuia e te NP19 ngā mahi peroxidase (POD) me te superoxide dismutase (SOD) mā te 6.1% ki te 63.0% me te 3.0% ki te 67.7%, ia, e tohu ana i te whakapakari ake i ngā tikanga tiaki tipu. Ki te whakatauritea ki ngā mana whakahaere NP19 kāore i pangia, i whakaatuhia e ngā tipu raihi i pangia e te NP19 he pikinga o te ihirangi tae mā te 0.3%–24.7%, te maha o ngā kākano katoa mō ia panicle mā te 4.1%, te hua o ngā kākano katoa mā te 26.3%, te taupū papatipu hua o te hua mā te 34.4%, me te ihirangi o te matū kakara 2-acetyl-1-pyrroline (2AP) mā te 10.1%. I roto i ngā tipu raihi i pangia e te NP19 me te pahūtanga, ko ngā pikinga he 0.2%–49.2%, 4.6%, 9.1%, 54.4%, me te 7.5% ia. I whakaatuhia e ngā whakamātautau mara he nui ake te maha o ngā kākano katoa i roto i ia panicle i ngā tipu raihi i whakatipuria, i werohia rānei ki te NP19, mā te 15.1–27.2%, te hua o te kākano katoa mā te 103.6–119.8%, me te nui o te 2AP mā te 18.0–35.8%. He nui ake hoki te mahi SOD i whakaatuhia e ēnei tipu raihi (6.9–29.5%) ki te whakataurite ki ngā tipu raihi i pangia e te pahūtanga kāore i werohia ki te NP19. Nā te paninga rau o te NP19 i muri i te pangia i whakaroa te haere whakamua o te whara. Nō reira, i whakaatuhia ko te K. oryziphila NP19 he matū koiora me he patu pepeke koiora pea hei whakatairanga i te tipu o te tipu hei whakahaere i te pahūtanga raihi.
Heoi, he maha ngā āhuatanga e pāngia ai te whai huatanga o ngā patu harore, tae atu ki te hanganga, te wā me te tikanga tono, te kaha o te mate, te whai huatanga o ngā pūnaha matapae mate, me te putanga mai o ngā momo ātete ki ngā patu harore. Hei tāpiri, ko te whakamahinga o ngā patu harore matū ka pāngia te taiao e te paitini, ā, ka tūpono hoki ki te hauora o ngā kaiwhakamahi.
I roto i te whakamātautau kohua, i whakahoromatahia te mata o ngā purapura raihi, ā, i whakapūpūtia kia rite ki te whakaahuatanga i runga ake nei. Kātahi ka whakatōkia ki te K. oryziphila NP19, ā, ka whakatōkia ki roto i ngā paepae tipu. I whakatōkia ngā tipu mō ngā rā 30 kia puta mai ai ngā tipu raihi. Kātahi ka whakatōkia ngā tipu ki roto i ngā kohua. I te wā o te whakatō, i whakawairakautia ngā tipu raihi hei whakarite i a rātou mō te pangia e te harore e puta ai te pahūtanga o te raihi, hei whakamātautau hoki i tō rātou ātete.
I roto i tētahi whakamātautau mara, i rongoatia ngā purapura kua pihi ake i pangia e te Aspergillus oryzae NP19 mā te whakamahi i te tikanga kua whakaahuatia i runga ake nei, ā, i wehea kia rua ngā rōpū: ngā purapura kua pangia e te Aspergillus oryzae NP19 (RS) me ngā purapura kāore i pangia (US). I whakatōkia ngā purapura kua pihi ake ki roto i ngā tirei me te oneone kua whakahoromatatia (he ranunga oneone, he kiri raihi kua tahuna, me te paru kararehe i te ōwehenga 7:2:1 mā te taumaha) ā, i whakatōkia mō ngā rā 30.
I tāpirihia te whakatārewatanga koni oryziphila ki te raihi R, ā, i muri i te 30 hāora o te whakatipu, i tāpirihia te 2 μl o te K. oryziphila NP19 ki te wāhi kotahi. I whakatipuria ngā rihi Petri katoa i te 25°C i roto i te pōuri mō ngā hāora 30, kātahi ka whakatipuria i raro i te whakamārama tonu. I tāruatia ia rōpū e toru ngā wā. I muri i te 72 hāora o te whakatipu, i tirotirohia ngā wāhanga tipu, ā, i tukuna ki te karu hiko matawai. I roto i te poto, i whakapirihia ngā wāhanga tipu ki roto i te tote phosphate-buffered kei roto ko te 2.5% (v/v) glutaraldehyde, ā, i whakamaroketia ki roto i te raupapa o ngā otinga etanoru. I whakamaroketia ngā tauira ki te hauhā waro, kātahi ka pania ki te koura, ā, i tirohia i raro i te karu hiko matawai mō ngā meneti 15.
Wā tuku: Oketopa-13-2025