He pūmua matua tiakina ngā pūmua DELLAngā kaiwhakahaere tipuhe mahi matua tā rātou ki te whakahaere i te whanaketanga o te tipu hei whakautu ki ngā tohu ā-roto me te taiao. He kaiwhakahaere whakawhitiwhiti kōrero a DELLA, ā, ka tohua hei aro ki ngā kaiwhakatairanga mā te herea ki ngā take whakawhitiwhiti kōrero (TF) me te histone H2A mā roto i tōna rohe GRAS. Kua whakaatuhia e ngā rangahau tata nei ko te pumau o DELLA ka whakahaeretia i muri i te whakamaoritanga mā roto i ngā tikanga e rua: te polyubiquitination i puta mai i te phytohormone gibberellin, e arahi ana ki tōna tere whakahekenga, me te hononga o ngā whakarerekētanga iti rite ki te ubiquitin (SUMO) hei whakanui ake i tōna kohikohinga. Hei tāpiri, ko te mahi a DELLA ka whakahaeretia hihiritia e ngā glycosylations rerekē e rua: ka whakarei ake te taunekeneke DELLA-TF e te O-fucosylation engari ka aukatia e te whakarerekētanga O-linked N-acetylglucosamine (O-GlcNAc). Heoi, kāore anō kia mārama te mahi a te phosphorylation DELLA, nā te mea kua whakaatuhia e ngā rangahau o mua he hua taupatupatu, mai i ngā mea e whakaatu ana ko te phosphorylation ka whakatairanga, ka whakaiti rānei i te whakahekenga o DELLA ki ētahi atu e whakaatu ana kāore te phosphorylation e pā ki tōna pumau. I konei, ka tautuhia e mātou ngā pae phosphorylation i roto i te REPRESSORga1-3(RGA, AtDELLA) i purea mai i Arabidopsis thaliana mā te tātaritanga irahiko papatipu, ā, e whakaatu ana ko te phosphorylation o ngā peptide RGA e rua i ngā rohe PolyS me PolyS/T e whakatairanga ana i te herenga H2A me te whakarei ake i te mahi RGA. Te hononga o te RGA ki ngā kaiwhakatairanga ūnga. He mea nui, kāore te phosphorylation e pā ki ngā taunekeneke RGA-TF, ki te pumau RGA rānei. E whakaatu ana tā mātou rangahau i te tikanga ngota e whakaoho ai te phosphorylation i te mahi DELLA.
Hei whakamārama i te mahi a te phosphorylation i roto i te whakahaere i te mahi a DELLA, he mea nui kia tautuhia ngā pae phosphorylation a DELLA i roto i te tinana ora, me te mahi i ngā tātaritanga mahi i roto i ngā tipu. Mā te purenga hononga o ngā tangohanga tipu, ā, whai muri ko te tātaritanga MS/MS, i kitea e mātou ētahi pae phospho i roto i te RGA. I raro i ngā āhuatanga o te korenga o te GA, ka piki ake te phosphorylation RHA, engari kāore te phosphorylation e pā ki tōna pumau. He mea nui, i whakaatuhia e ngā whakamātautau co-IP me ChIP-qPCR ko te phosphorylation i te rohe PolyS/T o RGA e whakatairanga ana i tana taunekeneke ki te H2A me tana hononga ki ngā kaiwhakatairanga ūnga, e whakaatu ana i te tikanga e whakaoho ai te phosphorylation i te mahi a te RGA.
Ka tohua te RGA ki te chromatin ūnga mā te taunekeneke o te rohe LHR1 ki te TF, kātahi ka herea ki te H2A mā tōna rohe PolyS/T me te rohe PFYRE, ka hanga i te matatini H2A-RGA-TF hei whakau i te RGA. Ko te phosphorylation o Pep 2 i roto i te rohe PolyS/T i waenga i te rohe DELLA me te rohe GRAS mā te kinase kāore i te mōhiotia ka whakarei ake i te herenga RGA-H2A. Ka whakakorea e te pūmua mutant rgam2A te phosphorylation RGA, ā, ka whakamahia he āhua pūmua rerekē hei whakararuraru i te herenga H2A. Ka hua ake tēnei i te kore pumau o ngā taunekeneke TF-rgam2A wā poto me te wehenga o te rgam2A mai i te chromatin ūnga. E whakaatu ana tēnei pikitia i te pehitanga whakawhiti ira-ā-RGA anake. Ka taea te whakaahua i tētahi tauira rite mō te whakahohenga whakawhiti ira-ā-RGA, engari ko te matatini H2A-RGA-TF ka whakatairanga i te whakawhiti ira ūnga, ā, ka whakaitihia te whakawhiti ira-ā-rgam2A. I whakarerekētia te pikitia mai i a Huang et al.21.
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Kua tukuna ngā raraunga proteomics mō te ine ira papatipu ki te rōpū ProteomeXchange mā te putunga hoa PRIDE66 me te tohu raraunga PXD046004. Ko ngā raraunga katoa i whiwhihia i tēnei rangahau kei roto i ngā Mōhiohio Tāpiri, ngā Kōnae Raraunga Tāpiri, me ngā Kōnae Raraunga Mata. Kua whakaratohia ngā raraunga pūtake mō tēnei tuhinga.
Wā tuku: Whiringa-ā-rangi-08-2024