Ka tiakina ngā pūmua DELLAngā kaiwhakahaere tipue whai wāhi nui ana ki te whanaketanga o te tipu hei whakautu ki ngā tohu ā-roto, ā-waho hoki. Hei kaiwhakahaere whakawhiti kōrero, ka herea ngā DELLA ki ngā take whakawhiti kōrero (TF) me te histone H2A mā roto i ō rātou rohe GRAS, ā, ka tohua hei mahi ki ngā kaiwhakatairanga. Kua whakaatuhia e ngā rangahau tata nei ko te pumau o DELLA ka whakahaeretia i muri i te whakamaoritanga e ngā tikanga e rua: te polyubiquitination i puta mai i te homoni tipute kiperelini, e arahi ana ki tō rātou tere pirau, me te hononga ki te whakarerekētanga iti rite ki te ubiquitin (SUMO), e whakanui ana i tō rātou kohikohinga. Heoi anō, ko te mahi a DELLA e whakahaerehia ana e ngā tikanga glycosylation motuhake e rua: Ko te O-fucosylation e whakarei ake ana i te taunekeneke DELLA-TF, ko te whakarerekētanga O-linked N-acetylglucosamine (O-GlcNAc) e aukati ana i te taunekeneke DELLA-TF. Heoi anō, kāore i te mārama te tūranga o te phosphorylation DELLA i te mea kua whakaatuhia e ngā rangahau o mua he hua taupatupatu, ko ētahi e kī ana ko te phosphorylation e whakatairanga ana, e pehi ana rānei i te pirau o DELLA, ko ētahi e kī ana kāore te phosphorylation e pā ki tō rātou pumau. I konei, ka tautuhia e mātou ngā pae phosphorylation i roto i te GA1-3 repressor (RGA), AtDELLA, i purea mai i Arabidopsis thaliana mā te spectrometry papatipu, ā, e whakaatu ana ko te phosphorylation o ngā peptide RGA e rua i roto i ngā rohe PolyS me PolyS/T e whakarei ake ana i te mahi RGA mā te whakatairanga i te here H2A me te hononga RGA ki ngā kaiwhakatairanga ūnga. He mea nui, kāore te phosphorylation i pā ki ngā taunekeneke RGA-TF, ki te pumau RGA rānei. E whakaatu ana tā mātou rangahau i tētahi tikanga ngota e whakaoho ai te phosphorylation i te mahi DELLA.
I whakaatuhia e tā mātou tātaritanga irahiko papatipu he nui te phosphorylation o Pep1 me Pep2 i roto i te RGA i roto i te papamuri Ga1 ngoikore-GA. Haunga tēnei rangahau, kua whakaatuhia hoki e ngā rangahau phosphoproteomic te phosphorylation o Pep1 i roto i te RGA, ahakoa kāore anō kia rangahauhia tōna tūranga53,54,55. He rerekē, kāore anō kia whakaahuahia te phosphorylation o Pep2 i mua i te mea ka taea anake te kite i tēnei peptide mā te whakamahi i te ira whakawhiti RGAGKG. Ahakoa he iti noa te whakaiti a te whakarerekētanga m1A, i whakakore i te phosphorylation o Pep1, i te mahi RGA i roto i te tipu, he pānga tāpiri i te wā i honoa ai ki te m2A ki te whakaiti i te mahi RGA (Pikitia Tāpiri 6). He mea nui, he nui te whakaiti o te phosphorylation o Pep1 i roto i te whakarerekētanga sly1 whakarei ake-GA ki te whakataurite ki te ga1, e kī ana ko te GA e whakatairanga ana i te dephosphorylation o te RGA, e whakaiti ana i tana mahi. Ko te tikanga e pehi ai a GA i te phosphorylation o te RGA me rangahau anō. Ko tētahi pea ko tēnei ka tutuki mā te whakahaere i te kinase pūmua kāore i te mōhiotia. Ahakoa kua whakaatuhia e ngā rangahau ka whakahekehia te whakaaturanga o te kinase pūmua CK1 EL1 e te GA i roto i te raihi41, e whakaatu ana ā mātou hua kāore ngā whakarerekētanga taumata teitei o te Arabidopsis EL1 homologue (AEL1-4) e whakaiti i te phosphorylation RGA. E rite ana ki ā mātou hua, kāore i kitea e tētahi rangahau phosphoproteomic tata nei e whakamahi ana i ngā raina Arabidopsis AEL overexpressing me te ael triple mutant tētahi pūmua DELLA hei substrates mō ēnei kinases56. I te wā i whakaritea ai e mātou te tuhinga, i pūrongohia ko GSK3, te ira e whakamārama ana i te kinase GSK3/SHAGGY-rite i roto i te witi (Triticum aestivum), ka taea te phosphorylate i a DELLA (Rht-B1b)57, ahakoa kāore anō kia whakaūtia te phosphorylation o Rht-B1b e GSK3 i roto i te planta. Ko ngā tauhohenga enzymatic in vitro i te aroaro o GSK3 i whai muri i te tātaritanga spectrometry papatipu i whakaatu i ngā pae phosphorylation e toru kei waenganui i ngā rohe DELLA me GRAS o Rht-B1b (Pikitia Tāpiri 3). Nā ngā whakakapinga serine ki te alanine i ngā pae phosphorylation e toru i heke ai te mahi Rht-B1b i roto i te witi transgenic, e rite ana ki ā mātou kitenga i whakaitihia e ngā whakakapinga alanine i roto i te Pep2 RGA te mahi RGA. Heoi, i whakaatuhia anō e ngā whakamātautau whakahekenga pūmua in vitro ka taea hoki e te phosphorylation te whakau i te Rht-B1b57. He rerekē tēnei ki ā mātou hua e whakaatu ana kāore ngā whakakapinga alanine i roto i te Pep2 RGA e whakarerekē i tōna pumau i roto i te planta. Ko te GSK3 i roto i te witi he ortholog o te brassinosteroid-insensitive protein 2 (BIN2) i roto i te Arabidopsis 57. He kaiwhakahaere kino te BIN2 o te tohu BR, ā, ka whakahohehia e te BR tōna ara tohu mā te whakaputa i te whakahekenga BIN2 58. I whakaaturia e mātou kāore te maimoatanga BR e whakaiti i te pumau RGA 59, i ngā taumata phosphorylation rānei i roto i te Arabidopsis (Pikitia Tāpiri 2), e kī ana kāore pea te RGA e phosphorylated e BIN2.
I tātarihia ngā raraunga tauanga katoa mā te whakamahi i te Excel, ā, i whakatauhia ngā rerekētanga nui mā te whakamahi i te whakamātautau-t a Student. Kāore i whakamahia he tikanga tatauranga hei whakatau i te rahi o te tauira. Kāore he raraunga i tangohia mai i te tātari; kāore i matapōkeretia te whakamātautau; ā, i mōhio ngā kairangahau ki te tohatoha i te wā o te whakamātautau me te aromatawai putanga. Kei roto i ngā pūrākau ahua me ngā kōnae raraunga mata ngā rahi tauira.
Wā tuku: 15 Paenga-whāwhā 2025