Kua kitea e ngā kairangahau o te Tari Matū Koiora i te Indian Institute of Sciences (IISc) tētahi tikanga kua roa e rapuhia anahei whakahaere i te tipu o ngā tipu whenua taketake pērā i ngā bryophytes (he rōpū kei roto ko ngā mosses me ngā ateworts) i puritia i roto i ngā tipu pua o muri mai.
Ko te rangahau, i whakaputaina i roto i te puka Nature Chemical Biology, i arotahi ki te whakahaere kore-tikanga o ngā pūmua DELLA, he kaiwhakahaere tipu matua e pehi ana i te wehenga pūtau i roto i ngā embryophytes (ngā tipu whenua).
He mea whakamīharo, ko ngā bryophytes, arā, ngā tipu tuatahi i puta ki te whenua tata ki te 500 miriona tau ki muri, kāore i a rātou te kaiwhakawhiwhi GID1, ahakoa i hangaia e rātou te phytohormone GA. Ka ara ake te pātai mō te whakahaere i te tipu me te whanaketanga o ēnei tipu whenua o mua.
Mā te whakamahi i te pūwero ate o te Marchantia polymorpha hei tauira pūnaha, i kitea e ngā kairangahau he whākōkī motuhake tā ēnei tipu taketake, ko te MpVIH, e whakaputa ana i te inositol pyrophosphate karere pūtau (InsP₈), hei wawahi i a DELLA me te kore e whai wāhi mai ate waikawa gibberellic.
I whakamahia e ngā kairangahau te pūnaha CRISPR-Cas9 hei whakakore i te ira e whakamārama ana i te whākōkī VIH, hei whakaū i tana mahi. Ko ngā tipu kāore i te mahi VIH i whakaatu i ngā hapa whanaketanga nui me ngā rerekētanga āhua, pērā i ngā rau kiato, te ngoikore o te tipu radial, me te kore o ngā calyx. I whakakorea ēnei hapa mā te whakarerekē i te ira tipu hei whakaputa i te pito kotahi anake (te N-terminus) o te whākōkī VIH. Mā te whakamahi i ngā tikanga chromatography matatau, i kitea e te tīma kei roto i te N-terminus he rohe kinase e whakaoho ana i te hanga o InsP₈.
I kitea e ngā kairangahau ko DELLA tētahi o ngā pūtau e whai pānga ana ki te VIH kinase. I tua atu, i kitea e rātou he rite ngā momo āhua o ngā tipu ngoikore-MpVIH ki ērā o ngā tipu M. polymorpha me te pikinga ake o te whakaaturanga DELLA.
"I tēnei wā, i harikoa mātou ki te mārama mēnā kei te piki ake te pumau, te ngohe rānei o DELLA i roto i ngā tipu ngoikore-MpVIH," te kī a Priyanshi Rana, te kaituhi tuatahi me te tauira paerua i roto i te rōpū rangahau a Lahey. E rite ana ki tā rātou whakapae, i kitea e ngā kairangahau ka taea e te aukati DELLA te whakaora i ngā momo tipu me te whanaketanga hapa o ngā tipu whakarerekētanga MpVIH. E kī ana ēnei hua he kino te whakahaere a te VIH kinase i te DELLA, e whakatairanga ana i te tipu me te whanaketanga o ngā tipu.
I tīmata te rangahau mō ngā pūmua DELLA i te Huringa Kakariki, i te wā i whakamahia ai e ngā kaipūtaiao tō rātou pūmanawa ki te waihanga i ngā momo hua iti-iti. Ahakoa kāore i te mārama ngā taipitopito o tā rātou mahi i taua wā, mā te hangarau hou ka taea e ngā kaipūtaiao te whakarerekē i ngā mahi a ēnei pūmua mā te hangarau ira, ka piki ake ai ngā hua o te hua.
Wā tuku: Oketopa-23-2025