Kua kitea e ngā kairangahau o te Tari Matū Koiora i te Indian Institute of Sciences (IISc) tētahi tikanga kua roa e rapua ana e whakamahia ana e ngā tipu whenua taketake pērā i ngā bryophytes (tae atu ki ngā mosses me ngā liverworts) ki tewhakahaere i te tipu o ngā tipu– he tikanga kua tiakina hoki i roto i ngā tipu puāwai hou kua whanake ake.
Ko te rangahau, i whakaputaina i roto i te puka Nature Chemical Biology, e arotahi ana ki te whakahaere kore-tūturu o te pūmua DELLA, he kaiwhakahaere tipu matua ka taea te aukati i te wehenga pūtau i roto i ngā tipu kukune (ngā tipu whenua).
"He rite te mahi a DELLA ki te pūwero tere, engari ki te noho tonu tēnei pūwero tere, kāore e taea e te tipu te neke," te whakamārama a Debabrata Laha, he ahorangi tuarua mō te matū koiora me te kaituhi tahi o te rangahau. Nō reira, he mea nui te whakapōrearea o ngā pūmua DELLA hei whakatairanga i te tipu o ngā tipu. I roto i ngā tipu puāwai, ka whakapōrearea a DELLA ina te phytohormonete kiperelini (GA)ka herea ki tana kaiwhakawhiwhi GID1, ka hanga i te matatini GA-GID1-DELLA. Muri iho, ka herea te pūmua aukati DELLA ki ngā mekameka ubiquitin, ā, ka whakahoroa e te proteasome 26S.
He mea whakamīharo, ko ngā bryophytes ētahi o ngā tipu tuatahi ki te noho ki te whenua, tata ki te 500 miriona tau ki muri. Ahakoa e whakaputa ana rātou i te phytohormone gibberellin (GA), kāore i a rātou te GID1 receptor. Nā tēnei ka ara ake te pātai: me pēhea te whakahaere i te tipu me te whanaketanga o ēnei tipu whenua o mua?
I whakamahia e ngā kairangahau te pūnaha CRISPR-Cas9 hei patu i te ira VIH e rite ana, ā, nā reira i whakaū ai i te mahi a VIH. Ko ngā tipu kāore he whākōkī VIH mahi e whakaatu ana i ngā hapa tipu me te whanaketanga nui me ngā rerekētanga āhua, pērā i te thallus matotoru, te tipu radial ngoikore, me te kore o te calyx. I whakatikatikaina ēnei hapa mā te whakarerekē i te ira tipu hei whakaputa i te pito kotahi anake (te N-terminus) o te whākōkī VIH. Mā te whakamahi i ngā tikanga chromatography matatau, i kitea e te tīma rangahau kei roto i te N-terminus he rohe kinase e whakaoho ana i te hanga o InsP₈.
I kitea e ngā kairangahau ko DELLA tētahi o ngā pūtau e whai pānga ana ki te VIH kinase. I tua atu, i kitea e rātou he rite te āhua o ngā tipu ngoikore-MpVIH ki te āhua o ngā tipu Miscanthus multiforme me te pikinga ake o te whakaaturanga DELLA.
"I tēnei wā, kei te hiahia mātou ki te whakatau mēnā kei te piki ake te pumau, te ngohe rānei o DELLA i roto i ngā tipu ngoikore-MpVIH," te kī a Priyanshi Rana, he tauira tākuta i roto i te rōpū rangahau a Lahey me te kaituhi tuatahi o te pepa. E rite ana ki tā rātou whakapae, i kitea e ngā kairangahau he nui te whakahokinga mai o te aukati DELLA i ngā hapa tipu me te whanaketanga i roto i ngā tipu kua whakarerekētia e te MpVIH. E kī ana ēnei kitenga he kino te whakahaere a te VIH kinase i te DELLA, ā, nā reira ka whakatairanga i te tipu me te whanaketanga o ngā tipu.
I whakakotahi ngā kairangahau i ngā tikanga ira, matū koiora, me te koiora ā-tinana hei whakamārama i te tikanga e whakahaere ai te inositol pyrophosphate i te whakaaturanga pūmua DELLA i roto i tēnei bryophyte. Ina koa, ko te InsP₈, i hangaia e MpVIH, ka herea ki te pūmua MpDELLA, ka whakatairanga i tōna polyubiquitination, ka arahi atu ki te pakaru o tēnei pūmua pehi e te proteasome.
I ahu mai ngā rangahau mō te pūmua DELLA i te Huringa Kakariki, i te wā i whakamahia ai e ngā kaipūtaiao tōna pūmanawa ki te waihanga i ngā momo hua iti-iti. Ahakoa kāore i te mōhiotia tōna tikanga mahi i taua wā, nā ngā hangarau hou i āhei ai ngā kaipūtaiao ki te whakamahi i te whakatika ira hei whakahaere i te mahi a tēnei pūmua, ā, nā reira ka piki ake ngā hua o te hua.
"I te tipu haere o te taupori me te whakaiti haere o te whenua ahuwhenua, kua tino hira te whakapiki i ngā hua o te hua," te kī a Raha. I te mea ka horapa te pirau o te DELLA e whakahaeretia ana e InsP₈ i roto i ngā tipu kua whānau, ka taea e tēnei kitenga te whakatakoto huarahi mō te whanaketanga o ngā hua hua nui o te whakatupuranga e whai ake nei.
Wā tuku: Oketopa-31-2025