Uniconazolehe triazolekaiwhakahaere tipu tipue whakamahia whānuitia ana hei whakahaere i te teitei o te tipu me te aukati i te tipu nui o te tipu. Heoi, kāore anō kia mārama te tikanga ngota e aukati ai te uniconazole i te roa o te hypocotyl o te tipu, ā, he torutoru noa ngā rangahau e whakakotahi ana i ngā raraunga transcriptome me te metabolome hei tirotiro i te tikanga o te roa o te hypocotyl. I konei, i kite mātou he nui te aukati a te uniconazole i te roa o te hypocotyl i roto i ngā tipu kāpeti pua Hainamana. He mea whakamīharo, i runga i te tātaritanga transcriptome me te metabolome kua whakakotahihia, i kitea e mātou he nui te pānga o te uniconazole ki te ara "phenylpropanoid biosynthesis". I roto i tēnei ara, kotahi noa te ira o te whānau ira whakahaere whākōkī, BrPAL4, e whai wāhi ana ki te biosynthesis lignin, i tino heke iho. Hei tāpiri, i whakaatuhia e ngā whakamātautau rewena kotahi-ranu me te rua-ranu ka taea e BrbZIP39 te here tika ki te rohe whakatairanga o BrPAL4 me te whakahohe i tana tuhinga. I whakamātauhia anō e te pūnaha whakamōmona ira i puta mai i te huaketo ka taea e BrbZIP39 te whakahaere pai i te roa o te hypocotyl o te kāpeti Hainamana me te hanganga hypocotyl lignin. Mā ngā hua o tēnei rangahau ka kitea he māramatanga hou mō te tikanga whakahaere ngota o te cloconazole ki te aukati i te roa o te hypocotyl o te kāpeti Haina. I whakaūtia mō te wā tuatahi i whakaitihia e te cloconazole te nui o te lignin mā te aukati i te hanganga phenylpropanoid i whakahaerehia e te kōwae BrbZIP39-BrPAL4, ā, ka hua ake te whakaiti i te hypocotyl i roto i ngā tipu kāpeti Haina.
Ko te kāpeti Haina (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) nō te puninga Brassica, ā, he huawhenua ripeka ā-tau rongonui e whakatipuria whānuitia ana i tōku whenua (Wang et al., 2022; Yue et al., 2022). I ngā tau tata nei, kua piki haere tonu te tauine whakaputa o te kāpeti Haina, ā, kua rerekē te tikanga whakatipu mai i te rui tika tuku iho ki te whakatipu tipu me te whakawhitinga tipu kaha. Heoi, i roto i te tukanga whakatipu tipu kaha me te whakawhitinga tipu, ko te tipu nui o te hypocotyl ka puta he tipu roa, ka hua ake he kounga tipu ngoikore. Nō reira, ko te whakahaere i te tipu nui o te hypocotyl he take nui i roto i te whakatipu tipu kaha me te whakawhitinga o te kāpeti Haina. I tēnei wā, he iti noa ngā rangahau e whakauru ana i ngā raraunga transcriptomics me te metabolomics hei tūhura i te tikanga o te roa o te hypocotyl. Kāore anō kia rangahaua te tikanga ngota e whakahaere ai te chlorantazole i te whakawhānui o te hypocotyl i roto i te kāpeti Haina. Ko tā mātou whāinga he tautuhi ko ēhea ira me ngā ara ngota e whakautu ana ki te whakaiti i te hypocotyl i puta mai i te uniconazole i roto i te kāpeti Haina. Mā te whakamahi i ngā tātaritanga transcriptome me te metabolomic, tae atu ki te tātaritanga yeast one-ranu, te whakamātautau luciferase takirua, me te whakamātautau whakangāwari ira i puta mai i te huaketo (VIGS), i kitea e mātou ka taea e te uniconazole te whakaoho i te hypocotyl dwarfing i roto i te kāpeti Haina mā te aukati i te biosynthesis lignin i roto i ngā tipu kāpeti Haina. Ka whakaratohia e ā mātou hua he māramatanga hou ki te tikanga whakahaere ngota e aukati ai te uniconazole i te roa o te hypocotyl i roto i te kāpeti Haina mā te aukati i te biosynthesis phenylpropanoid e whakahaerehia ana e te kōwae BrbZIP39–BrPAL4. Tērā pea he pānga mahi nui ēnei hua mō te whakapai ake i te kounga o ngā tipu arumoni me te whai wāhi ki te whakarite i te hua me te kounga o ngā huawhenua.
I whakaurua te BrbZIP39 ORF roa-katoa ki roto i te pGreenll 62-SK hei whakaputa i te kaiwhakaputa, ā, i honoa te kongakonga whakatairanga BrPAL4 ki te ira pūrongo pGreenll 0800 luciferase (LUC) hei whakaputa i te ira pūrongo. I whakawhitia ngātahitia ngā kawe ira kaiwhakaputa me te kaiwhakaputa hei rau tupeka (Nicotiana benthamiana).
Hei whakamārama i ngā whanaungatanga o ngā metabolites me ngā ira, i whakahaerehia e mātou he tātaritanga ngātahi o te metabolome me te transcriptome. I whakaatuhia e te tātaritanga whakarei ake i te ara KEGG ko ngā DEG me ngā DAM i whakarei ake i roto i ngā ara KEGG e 33 (Pikitia 5A). I roto i aua ara, ko te ara "phenylpropanoid biosynthesis" te mea tino whakarei ake; ko te ara "photosynthetic carbon fixation", te ara "flavonoid biosynthesis", te ara "pentose-glucuronic acid interconversion", te ara "tryptophan metabolism", me te ara "starch-sucrose metabolism". I whakaatuhia e te mahere whakarōpū wera (Pikitia 5B) ko ngā DAM e hono ana ki ngā DEG i wehea ki ētahi wāhanga, ko ngā flavonoid te wāhanga nui rawa atu, e tohu ana ko te ara "phenylpropanoid biosynthesis" he mahi nui i roto i te hypocotyl dwarfism.
E kī ana ngā kaituhi i whakahaerehia te rangahau i te korenga o tētahi whanaungatanga arumoni, whanaungatanga pūtea rānei ka taea te whakamārama hei taupatupatu pānga pea.
Ko ngā whakaaro katoa o tēnei tuhinga nō te kaituhi anake, ā, kāore e whakaata i ngā tirohanga a ngā whakahaere hono, ngā kaiwhakaputa, ngā ētita, ngā kaiarotake rānei. Ko ngā hua katoa i arotakengia i roto i tēnei tuhinga, ngā kereme rānei a ō rātou kaihanga, kāore i te whakamanahia, i te tautokona rānei e te kaiwhakaputa.
Wā tuku: Maehe-24-2025