Hei tētahi o ngā ahotea nui o te taiao, ka tino whakararu te ahotea pāmahana iti i te tipu o ngā tipu, ā, ka pā kino ki te hua me te kounga o ngā hua. Ko te waikawa 5-Aminolevulinic (ALA) he mea whakahaere tipu e kitea whānuitia ana i roto i ngā kararehe me ngā tipu. Nā tōna whai huatanga nui, kāore e paitini, ā, he ngāwari hoki te pirau, e whakamahia whānuitia ana i roto i te tukanga ātete makariri o ngā tipu.
Heoi, ko te nuinga o ngā rangahau e pā ana ki te ALA e aro nui ana ki te whakahaere i ngā pito mutunga whatunga. Kāore i te mārama i tēnei wā te tikanga ngota motuhake o te mahi a te ALA i roto i te manawanui wawe o ngā tipu ki te makariri, ā, me nui ake te rangahau a ngā kaipūtaiao.
I te marama o Hānuere 2024, i whakaputa a Horticultural Research i tētahi pepa rangahau ko te taitara "5-Aminolevulinic Acid Enhances Cold Tolerance by Regulating the SlMYB4/SlMYB88-SlGSTU43 Reactive Oxygen Species Scavenging Module in Tomato" nā te tīma a Hu Xiaohui i te ahuwhenua me te ngahere o Te Whare Wānanga o Northwestern.
I roto i tēnei rangahau, i kitea te ira glutathione S-transferase SlGSTU43 i roto i te tōmato (Solanum lycopersicum L.). I whakaatuhia e ngā hua o te rangahau he kaha te whakaoho a te ALA i te whakaaturanga o SlGSTU43 i raro i te ahotea makariri. Ko ngā raina tōmato whakawhiti ira e whakaatu nui ana i te SlGSTU43 i whakaatu i te kaha nui ake o te kaha ki te kohi momo hāora tauhohe, ā, i whakaatu i te ātete nui ki te ahotea pāmahana iti, engari ko ngā raina SlGSTU43 ira whakarerekē he tairongo ki te ahotea pāmahana iti.
Hei tāpiritanga, i whakaatuhia e ngā hua rangahau kāore te ALA e whakanui ake i te manawanui o te momo mutant ki te ahotea pāmahana iti. Nō reira, e kī ana te rangahau he ira nui a SlGSTU43 i roto i te tukanga whakarei ake i te manawanui makariri i roto i te tōmato mā te ALA (Pikitia 1).
Hei tāpiritanga, i whakaūhia e tēnei rangahau mā te kitenga EMSA, Y1H, LUC me te ChIP-qPCR ka taea e SlMYB4 me SlMYB88 te whakahaere i te whakapuakanga o SlGSTU43 mā te herea ki te kaiwhakatairanga SlGSTU43. I whakaatuhia e ētahi atu whakamātautau kei te whai wāhi atu hoki a SlMYB4 me SlMYB88 ki te tukanga ALC mā te whakanui ake i te manawanui o te tōmato ki te ahotea pāmahana iti me te whakahaere pai i te whakapuakanga o SlGSTU43 (Pikitia 2). Ka whakaratohia e ēnei hua he tirohanga hou ki te tikanga e whakarei ake ai te ALA i te manawanui ki te ahotea pāmahana iti i roto i te tōmato.
Ētahi atu kōrero: Zhengda Zhang et al., Ka whakarei ake te waikawa 5-aminolevulinic i te manawanui makariri mā te whakahaere i te kōwae SlMYB4/SlMYB88-SlGSTU43 mō te kohikohi i ngā momo hāora tauhohe i roto i te tōmato, Horticulture Research (2024). DOI: 10.1093/haora/uhae026
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Wā tuku: Hurae-22-2024